This thread explains how researchers mapped mitochondria in the human brain, showing they vary by region and cell type. Using brain slices and RNA data, they created MitoBrainMap v1.0, a detailed map of mitochondrial content. This helps us understand how energy is used in the brain and may reveal new insights into brain function.
The brain is full of mitochondria!
But how many, and where?
Different types of mitochondria specialize for energy transformation and other functions
In MitoBrainMap v1.0 we provide the first maps of mitochondrial content and OxPhos capacity across the human brain
The first challenge in this project was to partition the frozen human brain into small pieces, from which we could measure mitochondrial enzyme activities
The solution was to physically voxelize the brain. Eugene Mosharov created small cubes of brain using a CNC cutter
In four different voxels, @AnnaMonzel and colleagues performed single-nucleus RNA sequencing to profile mitochondria in different cell types
We learnt that mitochondria are both specialized across cell types and brain regions
The human brain is absolutely beautiful!
@MichelTdS thought that we could use standard neuroimaging data, train a model to predict mitochondrial features, and project the results at the scale of the whole brain
Maybe the spatial distribution and functional properties of brain mitochondria tell us something about how different energy patterns are sensed or transduced in the brain? @msahsorin
@msahsorin
You’re absolutely right —this deserves far more attention.
Many of us (@mcer33, @MitoPsychoBio, @StuartHameroff, et al.) are working to better understand the source and propagation of endogenous biological light, especially its role in the brain.
Once we unravel the source and https://t.co/wXk5sz9K5T
The brain is full of mitochondria!
But how many, and where?
Different types of mitochondria specialize for energy transformation and other functions
In MitoBrainMap v1.0 we provide the first maps of mitochondrial content and OxPhos capacity across the human brainThe first challenge in this project was to partition the frozen human brain into small pieces, from which we could measure mitochondrial enzyme activities
The solution was to physically voxelize the brain. Eugene Mosharov created small cubes of brain using a CNC cutterIn four different voxels, @AnnaMonzel and colleagues performed single-nucleus RNA sequencing to profile mitochondria in different cell types
We learnt that mitochondria are both specialized across cell types and brain regionsThe human brain is absolutely beautiful!
@MichelTdS thought that we could use standard neuroimaging data, train a model to predict mitochondrial features, and project the results at the scale of the whole brainThe result is MitoBrainMap v1.0Research briefing with a bit of the backstory behind the paper 😀
Full text:So grateful to @janellison @BaszuckiGroup and NIH for supporting this work
We hope it sets the stage for expanding our understanding of the mind-mitochondria connection
Thanks to @curiousdrgeorge for handling this paper to publicationSome more thoughts about why this project was meaningful and where it may lead toAll data available here:Full text PDF of the article with supplemental material:Maybe the spatial distribution and functional properties of brain mitochondria tell us something about how different energy patterns are sensed or transduced in the brain? @msahsorin
yes
The brain is full of mitochondria!
But how many, and where?
Different types of mitochondria specialize for energy transformation and other functions
In MitoBrainMap v1.0 we provide the first maps of mitochondrial content and OxPhos capacity across the human brain ... The first challenge in this project was to partition the frozen human brain into small pieces, from which we could measure mitochondrial enzyme activities
The solution was to physically voxelize the brain. Eugene Mosharov created small cubes of brain using a CNC cutter ... In four different voxels, @AnnaMonzel and colleagues performed single-nucleus RNA sequencing to profile mitochondria in different cell types
We learnt that mitochondria are both specialized across cell types and brain regions ... The human brain is absolutely beautiful!
@MichelTdS thought that we could use standard neuroimaging data, train a model to predict mitochondrial features, and project the results at the scale of the whole brain ... The result is MitoBrainMap v1.0 ... Research briefing with a bit of the backstory behind the paper
Full text: ... So grateful to @janellison @BaszuckiGroup and NIH for supporting this work
We hope it sets the stage for expanding our understanding of the mind-mitochondria connection
Thanks to @curiousdrgeorge for handling this paper to publication ... Some more thoughts about why this project was meaningful and where it may lead to ... All data available here: ... Full text PDF of the article with supplemental material: ... Maybe the spatial distribution and functional properties of brain mitochondria tell us something about how different energy patterns are sensed or transduced in the brain? @msahsorin
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